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1. Practical principles and operating steps of pipettes
Practical principles and operating steps of pipettes The working principle of pipettes is mainly divided into two categories according to their working principles: gas piston pipettes and external piston pipettes. Air push: The working principle is to push the piston through the expansion and contraction of the spring, remove some air, use atmospheric pressure to suck in liquid, and then push the air to discharge the liquid by the piston. There is an air column between the liquid and the piston, which is suitable for conventional aqueous solutions. External piston type pipette (Positive separation): The working principle is to extract and dispense liquid through the continuous movement of the piston inside the pipette or suction head. The piston comes into direct contact with the liquid without an air column. This design is not only suitable for conventional aqueous solutions, but also particularly suitable for problematic liquids such as high viscosity, volatility, and foaming. The operation steps of a pipette include selecting the appropriate pipette tip: The pipette tip and pipette tip are equally important, and only high-quality pipette tips can fully reflect the accuracy of the pipette. It is recommended to use the manufacturers original suction head to ensure smooth and flawless tip and uniform height, thereby ensuring the reproducibility of experimental results. Choose a pipette that is suitable for the range: The optimal range for using a pipette is 30% to 100% of its maximum range. Using within this range can ensure the accuracy and precision of pipetting. Assembly of pipette tip: Insert the pipette tip vertically into the pipette tip, gently pre


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